Betty Lou is a young girl from Sesame Street who has been played over the years by various performers.

She is made from the Hot Pink Little Anything Muppet, much like Prairie Dawn. Prairie and Betty Lou are sometimes difficult to separate. For example, once used an image of Prairie for both her character page and Betty Lou's.[1] (As of December 2009, though, the site has replaced the picture with one of Betty Lou.) A 1994 document from the CTW archives also indicates the difference between the girls by their hair.[2] Additionally, when Fran Brill would perform Betty Lou, she would generally use a different voice for her than the one she would use for Prairie Dawn.

During the early years, Betty Lou was a minor character who was performed by whoever was available to perform her. She seemed to appear more in books than on the show. Apart from singing "I Want a Monster to Be My Friend" (credited on The Sesame Street Monsters! album as "Little Girl"), notable appearances included the song "Exercise" in 1983, and singing "Amigo" with her bilingual friend Carlos in a sketch from 1984, and serving as lead singer (backed by Grover and various Anythings). She was an extra in the song "Listen to the Bells" and, according to the Sesame Street 35 Years Anniversary Game, she once opened up a medical program for dolls called the "Betty Lou Clinic."